Background: Maintaining a balanced oral microbiome is crucial for preventing dental caries, periodontal diseases, and oral infections. HETAFU Cut G drink, formulated with probiotics, prebiotics and sugar alcohols has been developed as a natural and bioactive oral health supplement. This study evaluates its effects on key oral microorganisms, including Streptococcus mutans, Lactobacillus species, Actinomycetes species, and Candida species, over an 8-week period. Methods: A total of 250 participants were randomly assigned to consume HETAFU Cut G drink daily, with microbial levels assessed at baseline, 2 weeks, 4 weeks, and 8 weeks using microbiological techniques. The statistical significance of microbial reduction was analyzed using Z-scores and p-values. Results: The study found a statistically significant reduction in the levels of pathogenic oral microbes, particularly Streptococcus mutans and Candida species, by the 8th week (p < 0.05). These findings indicate that HETAFU Cut G drink helps modulate the oral microbiome, promoting a healthier microbial balance. Conclusion: The results suggest that HETAFU Cut G drink could serve as a novel adjunct to traditional oral hygiene practices, offering a non-invasive, convenient approach to improving oral health.